Validation of MinION sequencing using the Illumina MiSeq platform was consistent with the results obtained with the MinION. A metagenome on environmental ice wedge soil samples was completed, with base calling and uplink/downlink carried out via satellite internet. The MAM plate successfully identified an active community capable of L-serine metabolism, which was used for metagenomic sequencing with the MinION to identify the active and enriched community. (96% closest similarity in GenBank) which we partially genome sequenced using the MinION. We obtained 39 microbial isolates using the cryo-iPlate, which included several putatively novel strains based on the 16S rRNA gene, including a Pedobacter sp. The life detection methods used here include (1) the cryo-iPlate for culturing microorganisms using diffusion of in situ nutrients into semi-solid media (2) a Microbial Activity Microassay (MAM) plate (BIOLOG Ecoplate) for detecting viable extant microorganisms through a colourimetric assay, and (3) the Oxford Nanopore MinION for nucleic acid detection and sequencing of environmental samples and the products of MAM plate and cryo-iPlate. Here, we describe in situ life detection and sequencing in the field in soils over laying ice wedges in polygonal permafrost terrain on Axel Heiberg Island, located in the Canadian high Arctic (79☂6′N), an analog to the polygonal permafrost terrain observed on Mars. Significant progress is being made in the development of the next generation of low cost life detection instrumentation with much smaller size, mass and energy requirements. 2Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, United States.
1Department of Natural Resource Sciences, McGill University, Ste.Goordial 1,2 *, Ianina Altshuler 1, Katherine Hindson 1, Kelly Chan-Yam 1, Evangelos Marcolefas 1 and Lyle G.
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